Title :
Analysis of urea in human serum using an oblique angle deposited Ag nanorod surface enhanced Raman scattering substrate
Author :
Han, Yun-ah ; Ju, Jonghyun ; Yoon, Yoosik ; Kim, Seok-min
Author_Institution :
Sch. of Mech. Eng., Chung-Ang Univ., Seoul, South Korea
Abstract :
We applied surface enhanced Raman spectroscopy (SERS) for the detection of urea in human serum. An oblique angle deposition method was used to prepare SERS-active substrates consisting of Ag nanorods. To maximize the SERS enhancement, the effects of Ag nanorod length on the SERS signal were analyzed. The SERS signals of different concentrations of urea solutions were measured in order to generate a regression model for use in analyzing the amount of urea in body fluid using the SERS substrate. To examine the feasibility of the fabricated SERS substrate, the amount of urea in human serum was measured using the SERS substrate and compared with that determined via conventional blood analysis.
Keywords :
biochemistry; biological techniques; biomedical measurement; blood; molecular biophysics; nanofabrication; nanorods; physiological models; proteins; regression analysis; silver; surface enhanced Raman scattering; Ag; SERS signals; SERS-active substrates; body fluid; conventional blood analysis; human serum; oblique angle deposited nanorod; oblique angle deposition method; regression model; surface enhanced Raman scattering substrate; urea analysis; urea detection; urea solution concentrations; Biochemistry; Humans; Monitoring; Photonics; Surface treatment;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on
Conference_Location :
Birmingham
Print_ISBN :
978-1-4673-2198-3
DOI :
10.1109/NANO.2012.6321979
