In situ fabricated porous filter - characterization and biological applications

Author

Moorthy, Jaisree ; Beebe, David J.

Author_Institution

Dept. of Biomed. Eng., Wisconsin Univ., Madison, WI, USA

fYear

2002

fDate

6/24/1905 12:00:00 AM

Firstpage

514

Lastpage

517

Abstract

Porous filter was fabricated inside a microchannel by phase separation photo-polymerization. The porosity of the filter varies with the composition of the prepolymer mixture. Four different filter compositions varying in water and crosslinker concentration was characterized using confocal microscopy and resistance measurements. Preliminary studies showed that the porous filter can separate blood cells from whole blood and the large surface area can be utilized for protein detection assays

Keywords

biological techniques; blood; cellular biophysics; filtration; flow through porous media; microfluidics; optical microscopy; phase separation; polymerisation; porous materials; proteins; separation; biological applications; biological assay; blood cells separation; confocal microscopy; crosslinker concentration; filter porosity; flow resistance; fluorescent-labeled beads; in situ fabricated porous filter; large surface area; microchannel; phase separation photopolymerization; prepolymer mixture composition dependence; protein adhesion; protein detection assays; water concentration; whole blood; Adhesives; Blood; Cells (biology); Electrical resistance measurement; Filters; Microchannel; Microscopy; Polymers; Proteins; Surface resistance;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on

Conference_Location

Madison, WI

Print_ISBN

0-7803-7480-0

Type

conf

DOI

10.1109/MMB.2002.1002394

Filename

1002394