Fluorescence anisotropy of protein - Gold nanoclusters

Author

Li, Chenhao ; Sutter, Jens U. ; Birch, David J S ; Chen, Yu

Author_Institution

Dept. of Phys., Strathclyde Univ., Glasgow, UK

fYear

2012

Firstpage

1

Lastpage

4

Abstract

Red-emitting gold nanoclusters (BSA-Au₂₅) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25μs. Fluorescence anisotropy measurements of BSA-Au25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.

Keywords

atomic clusters; biological techniques; fluorescence; fluorescence spectroscopy; gold; molecular biophysics; molecular configurations; nanobiotechnology; nanoparticles; pH; proteins; radiative lifetimes; Au₂₅; BSA-Au25; bovine serum albumin; fluorescence anisotropy; fluorescence lifetime; pH 3.1; pH 7; protein conformational changes; protein-gold nanoclusters; red emitting gold nanoclusters; time 1.25 mus; Color; Detectors; Fluorescence; Gold; Green products; Probes; Proteins; BSA; fluorescence anisotropy; gold nanoclusters; protein conformational change;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on

Conference_Location

Birmingham

ISSN

1944-9399

Print_ISBN

978-1-4673-2198-3

Type

conf

DOI

10.1109/NANO.2012.6322067

Filename

6322067