Title :
Microfluidics applied to Love-wave devices to detect biological warfare agents in dynamic mode
Author :
Matatagui, Daniel ; Fontecha, Jesus ; Fernandez, M.J. ; Santos, Jose Pedro ; Horrillo, M.C. ; Gracia, I. ; Cane, C.
Author_Institution :
GRIDSEN, Inst. de Seguridad de la Inf., Madrid, Spain
Abstract :
A Love-wave device combined with a microfluidic chip of PDMS has been developed to detect biological warfare agents (BWA) in liquid media. It has been tested with the realization of two immunoassays: in the first one the bacteriophage M13 has been detected through the mouse monoclonal antibody, anti-M13 (AM13) and in the second one the detection has been carried out by means of the immunoreaction of the rabbit immunoglobulin (Rabbit IgG) with the goat anti-rabbit polyclonal antibody (GAR). The detections have been performed in a dynamic mode (continuous flow-through) in order to obtain a short response time. Different concentrations have been detected with a fast response and a good discrimination among them.
Keywords :
Love waves; biosensors; cellular biophysics; microfluidics; microorganisms; AM13; BWA detection; GAR; Love-wave devices; PDMS; Rabbit IgG; antiM13; bacteriophage M13; biological warfare agent detection; continuous flow-through; dynamic mode; goat antirabbit polyclonal antibody; immunoassays; immunoreaction; liquid media; microfluidic chip; mouse monoclonal antibody; rabbit immunoglobulin; Biosensors; Immune system; Microchannel; Microfluidics; Rabbits; Time-frequency analysis; Acoustic wave sensor; Love wave; immunodetection biosensor; microfluidics;
Conference_Titel :
Electron Devices (CDE), 2013 Spanish Conference on
Conference_Location :
Valladolid
Print_ISBN :
978-1-4673-4666-5
Electronic_ISBN :
978-1-4673-4667-2
DOI :
10.1109/CDE.2013.6481356
