Detection of Vesicular Stomatitis Virus using a Capacitive Immunosensor

Author

Hanna, Darrin M. ; Gross, Brooks A. ; Kandlikar, Sachin ; Lempicki, Elizabeth ; Oakley, Barbara A. ; Stryker, Gabrielle A.

Author_Institution

Sch. of Eng. & Comput. Sci., Oakland Univ., Rochester, MI

fYear

2005

fDate

6/27/1905 12:00:00 AM

Firstpage

534

Lastpage

537

Abstract

This paper describes the detection of vesicular stomatitis virus using a novel capacitive immunosensor technology, whereby the respective antibodies for the antigens were used as a means for chemical detection on separate sensors. Devices were fabricated using standard etching and metal plating techniques, followed by immobilization of antibodies. Detection of antigen was performed by measuring voltage change due to changes in capacitance as antigen bound to the antibody surface. Capacitance changes were detected upon binding of specific antigen to the surface. This rugged, prototype device detected VSV down to the 2 pg/ml range

Keywords

biochemistry; bioelectric potentials; biosensors; capacitive sensors; chemical sensors; electroplating; etching; microorganisms; molecular biophysics; proteins; antibodies; antigens; capacitive immunosensor; chemical detection; etching; metal plating; vesicular stomatitis virus detection; voltage change; Capacitance measurement; Capacitive sensors; Chemical sensors; Chemical technology; Etching; Immune system; Performance evaluation; Prototypes; Sensor phenomena and characterization; Voltage measurement; Antigen; VSV; antibody; bacteria; capacitance; immunosensor; virus;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the

Conference_Location

Shanghai

Print_ISBN

0-7803-8741-4

Type

conf

DOI

10.1109/IEMBS.2005.1616466

Filename

1616466