Title :
A Micro Device for Separation of Erythrocytes and Leukocytes in Human Blood
Author :
Zheng, Siyang ; Tai, Yu-Chong ; Kasdan, Harvey
Author_Institution :
Caltech Micromachining Lab, California Inst. of Technol., Pasadena, CA
Abstract :
We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 mum lateral separation was achieved for 5 mum and 10 mum beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency
Keywords :
bioMEMS; biomedical equipment; biomedical measurement; blood; calibration; cellular biophysics; microchannel flow; particle size; separation; 10 mum; 420 mum; 5 mum; MEMS; PDMS cylindrical pillars; blood count; calibration; critical particle size; erythrocytes; fluidic chamber; glass slides; human blood; lateral displacement; leukocytes; micro device; polystyrene beads; red blood cells; separation; white blood cells; Calibration; Displacement measurement; Glass; Humans; Microelectromechanical devices; Particle measurements; Predictive models; Red blood cells; Size measurement; White blood cells;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616592
