DocumentCode :
1606255
Title :
Microfabricated Cell-based Biosensor Arrays
Author :
Pishko, Michael V.
Author_Institution :
Dept. of Chem. Eng., Pennsylvania State Univ., University Park, PA
fYear :
2006
Firstpage :
1058
Lastpage :
1064
Abstract :
Here, we described the fabrication using photolithography of poly(ethylene glycol) (PEG)-based hydrogel microstructures encapsulating viable mammalian cells on glass and silicon substrates. Substrates were treated with 3-(trichlorosilyl)propyl methacrylate to form pendant acrylate group to covalent link the hydrogel microstructure. Cells were encapsulated in arrays of cylindrical hydrogel microstructures 600 and 50 mum in diameter and viability assays demonstrated that encapsulated cells remained viable after photoencapsulation. These microstructures had clearly defined, three-dimensional structure without any residual cells remaining surface and no delamination of hydrogel elements from functionalized substrate occurred in aqueous environment for over a week. By changing spin-coating rates and feature sizes of photomasks, we could create cell-containing microstructures with aspect ratios ranging from 0.12 to 1.4. In case of 50 mum hydrogel microstructure, number of cells could be limited to 1 or 2 cells per element and array consisting of 400 elements could be fabricated in a square of 2 mm2. These cell-containing hydrogel microstructures were also successfully fabricated in poly(dimethylsiloxane) microchannels to create optical biosensor arrays of individually addressable single or multiple cell-containing hydrogel microstructures with potential applications in drug screening or pathogen detection
Keywords :
biomedical equipment; biosensors; cellular biophysics; delamination; drugs; masks; optical sensors; photolithography; polymers; spin coating; 3-(trichlorosilyl)propyl methacrylate; 50 mum; 600 mum; Si; SiO2; aqueous environment; aspect ratio; cell-based biosensor arrays; cell-containing microstructures; covalent link; cylindrical microstructures; drug screening; glass substrate; hydrogel microstructures; microchannels; microfabrication; optical biosensor arrays; pathogen detection; pendant acrylate group; photoencapsulation; photolithography; photomasks; poly(ethylene glycol); silicon substrate; spin-coating; three-dimensional structure; viability assays; viable mammalian cells; Biomedical optical imaging; Biosensors; Delamination; Fabrication; Glass; Lithography; Microchannel; Microstructure; Optical arrays; Silicon;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
Type :
conf
DOI :
10.1109/IEMBS.2005.1616601
Filename :
1616601
Link To Document :
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