Bienzymatic direct electrochemistry and electrocatalysis based on co-immobilization of glucose oxidase and cytochrome c on glassy carbon electrode modified by Chitosan film

In this paper, it was found that glucose oxidase (GOD) and cytochrome C (Cyt C) have been stably immobilized on the surface of glassy carbon electrode modified by chitosan (Chi) film. The sorption behavior of GOD and Cyt C immobilized on Chi matrix was characterized by environment scanning electron microscope (ESEM) which demonstrated that GOD and Cyt C have completely adsorbed the surface of Chi film. The direct electrochemistry and electrocatalysis performance of GOD and Cyt C on bienzymatic modified electrode were showed by cyclic voltammogram (CV) which indicated that GOD and Cyt C immobilized on Chi film display direct, quasi-reversible and surface-controlled electrochemical reaction containing proton exchange in 0.1M phosphate buffer solution (PBS) (pH 7.1), respectively. Furthermore, it was also discovered that GOD and Cyt C on modified electrode can show sensing performance to glucose and hydrogen peroxide, severally. Thus, single GOD-Cyt C/Chi/GC bienzymatic modified electrode is hopeful to be used in glucose biosensor or hydrogen peroxide biosensor.