Title :
Capacitive monitoring of the antigen-antibody reactions enhanced by nanogold
Author :
Guo, X.S. ; Fang, X.S. ; Yang, X.L. ; Chen, Y.Q. ; Wang, L.R.
Author_Institution :
Dept. of Biomed. Eng., Zhejiang Univ., Hangzhou
fDate :
6/27/1905 12:00:00 AM
Abstract :
An immunosensor for measuring antigen-antibody binding by nanogold particles enhancement was presented here. A gold plate electrode was modified by a SAM (self-assembled monolayer) of 11-mercaptoundecanioc acid. Then sheep anti-rabbit IgG was immobilized onto the end of the 11-mercaptoundecanioc acid. With that the gold electrode was exposed to the mixed solution of rabbit IgG and nanogold labeled sheep anti-rabbit IgG. The differential capacitance of the electrical double layer was monitored to detect the antigen-antibody reaction. The result showed the capacity of the electrical double layer by nanogold enhancement was largely increased compared to that in traditional capacitive immunosensor. The detection sensitivity is up to 10nF/mug/ml
Keywords :
biochemistry; capacitive sensors; electrochemical electrodes; electrochemistry; gold; microsensors; monolayers; nanobiotechnology; nanoparticles; self-assembly; 11-Mercaptoundecanioc Acid; Au; SAM; antigen-antibody reactions; capacitive monitoring; electrical double layer; electrode; immunosensor; nanogold particles; self-assembled monolayer; sheep anti-rabbit IgG; Biomedical electrodes; Biomedical engineering; Biosensors; Capacitance; Gold; Immune system; Monitoring; Nanobioscience; Rabbits; Self-assembly;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616654
