Investigation of interactions between two monoclonal antibodies and SARS virus with a Label-free Protein Array

Author

Qi, C. ; Chen, Y.-Y. ; Wang, Z.-H. ; Jin, G.

Author_Institution

Inst. of Mechanics, Chinese Acad. of Sci., Beijing

fYear

2006

Firstpage

1305

Lastpage

1308

Abstract

The investigation of interactions between two kinds of monoclonal antibodies and SARS virus with a label-free protein array technique were presented in this paper. The performance consists of three parts: a surface modification for ligand immobilization/surface, a protein array fabrication with an integrated microfluidic system for patterning, packaging and liquid handling, and a protein array reader of imaging ellipsometer. This revealed the technique could be used as an immunoassay for qualitative and quantitative detection as well as kinetic analysis of biomolecule interaction

Keywords

arrays; biochemistry; biosensors; ellipsometers; microfluidics; microorganisms; molecular biophysics; patient diagnosis; proteins; SARS virus; Si; biomolecule interaction; imaging ellipsometer; immunoassay; integrated microfluidic system; kinetic analysis; label-free protein array; ligand immobilization; liquid handling; monoclonal antibodies; packaging; patterning; protein array fabrication; protein array reader; surface modification; Biosensors; Ellipsometry; Fabrication; Kinetic theory; Microfluidics; Molecular biophysics; Optical imaging; Packaging; Proteins; Visualization; Protein array; SARS; antibody;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the

Conference_Location

Shanghai

Print_ISBN

0-7803-8741-4

Type

conf

DOI

10.1109/IEMBS.2005.1616666

Filename

1616666