Title :
Investigation of interactions between two monoclonal antibodies and SARS virus with a Label-free Protein Array
Author :
Qi, C. ; Chen, Y.-Y. ; Wang, Z.-H. ; Jin, G.
Author_Institution :
Inst. of Mechanics, Chinese Acad. of Sci., Beijing
Abstract :
The investigation of interactions between two kinds of monoclonal antibodies and SARS virus with a label-free protein array technique were presented in this paper. The performance consists of three parts: a surface modification for ligand immobilization/surface, a protein array fabrication with an integrated microfluidic system for patterning, packaging and liquid handling, and a protein array reader of imaging ellipsometer. This revealed the technique could be used as an immunoassay for qualitative and quantitative detection as well as kinetic analysis of biomolecule interaction
Keywords :
arrays; biochemistry; biosensors; ellipsometers; microfluidics; microorganisms; molecular biophysics; patient diagnosis; proteins; SARS virus; Si; biomolecule interaction; imaging ellipsometer; immunoassay; integrated microfluidic system; kinetic analysis; label-free protein array; ligand immobilization; liquid handling; monoclonal antibodies; packaging; patterning; protein array fabrication; protein array reader; surface modification; Biosensors; Ellipsometry; Fabrication; Kinetic theory; Microfluidics; Molecular biophysics; Optical imaging; Packaging; Proteins; Visualization; Protein array; SARS; antibody;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616666
