Title :
Quenching Properties of a Self-Referenced Fluorescence Oxygen Nanosensor under a Wide-Field Intrinsic Optical Signal Imaging System
Author :
Zhang, G. ; McShane, M.J. ; Robinson, C.J.
Author_Institution :
Biomedical Eng. Program, Louisiana Tech. Univ., Ruston, LA
Abstract :
Wide-field intrinsic optical signal (IOS) imaging is an excellent method to map in vivo neurological metabolic changes during brain activity. It has a spatial resolution of 50-100 mum and a wide imaging field of several cm2 that cannot be achieved by regular microscopic imaging. A nano-encapsulated fluorescence dye sensor that is specifically sensitive to oxygen level was fabricated by using electrostatic layer-by-layer (LbL) self-assembly technique. Fluorescence images of the nanosensor solution were collected by a wide-field IOS imaging system under different oxygen concentrations. Mathematical models for nanosensor fluorescence were developed based on the imaging results. Correlations between the nanosensor fluorescence and oxygen concentration models indicate an inverse quenching relationship between the nanosensor fluorescence and oxygen concentration
Keywords :
biomedical optical imaging; brain; fluorescence; gas sensors; nanotechnology; neurophysiology; oxygen; radiation quenching; O2; brain activity; electrostatic layer-by-layer self-assembly; fluorescence images; fluorescence oxygen nanosensor; neurological metabolic changes; optical signal imaging; oxygen concentration; quenching; Brain; Electrostatics; Fluorescence; In vivo; Microscopy; Optical imaging; Optical sensors; Oxygen; Self-assembly; Spatial resolution; Electrostatic layer-by-layer (LbL) self-assembly; Stern-Volmer equation; intrinsic optical signal (IOS) imaging; nanosensor;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616701
