Electrochemical characterization of DNA covered gold thin film electrodes for biosensoric applications

The scope of this work is to create different biologically active monolayers on gold thin film electrodes applying Self Assembled Monolayer (SAM) technology and to characterize them with electrochemical methods like Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). Three monolayer structures applying different methods that are used to gain better probe orientation were created and compared according to hybridization with complementary target DNA chains in two different lengths. An electrocleaning protocol to gain high quality electrode surfaces without the need of chemical cleaning reagents and a procedure to qualitatively monitor bioreceptor surfaces with good reproducibility were investigated.