DNA Fragmentation Induced by Intense Burst Sinusoidal Electric Fields

Author

Nomura, Naoyuki ; Yamamoto, Yuji ; Hayashi, Ryoichi ; Uto, Koki ; Katsuki, Sunao ; Akiyama, Hidenori ; Uchida, Ichiro ; Abe, Shin-ichi ; Takano, Hiroyoshi

Author_Institution

Univ. of Kumamoto, Kumamoto

fYear

2007

Firstpage

475

Lastpage

475

Abstract

Summary form only given. The objective of this paper is to investigate the DNA fragmentation by MHz range intense burst sinusoidal electric fields (IBSEF) by means of a single gel electrophoresis, Comet assay method. Although all IBSEF treated samples showed DNA fragmentation, there was no clear dependence on the frequencies. There are two possible mechanisms of the DNA fragmentation by IBSEF. One is the direct physical breaking of DNA. The other is an activation of biological process of DNA division, such as apoptosis. The agarose electrophoresis method indicates that DNA itself was not fragmented by the intense electric field with an amplitude of 4 kV/cm.

Keywords

DNA; bioelectric phenomena; biological effects of fields; biomolecular effects of radiation; cellular effects of radiation; electrophoresis; gels; genetics; Comet assay method; DNA division; DNA fragmentation; DNA strand breakage; IBSEF treated samples; agarose electrophoresis method; apoptosis; direct physical DNA breaking; intense burst sinusoidal electric fields; single gel electrophoresis; Bandwidth; Biological materials; Biological systems; Biomembranes; Cells (biology); DNA; Electrodes; Frequency dependence; Lipidomics; Platinum;

fLanguage

English

Publisher

ieee

Conference_Titel

Plasma Science, 2007. ICOPS 2007. IEEE 34th International Conference on

Conference_Location

Albuquerque, NM

ISSN

0730-9244

Print_ISBN

978-1-4244-0915-0

Type

conf

DOI

10.1109/PPPS.2007.4345781

Filename

4345781