Immobilization of Alkaline phosphatase on biopolymeric support

Author

Berteanu, A. Elena ; Tcacenco, Luminita ; Daniela Ionita, B. ; Demetrescu, Ioana

Author_Institution

Nat. Inst. of R&D for Biol. Sci., Bucharest, Romania

fYear

2014

fDate

13-15 Oct. 2014

Firstpage

43

Lastpage

46

Abstract

This study is an investigation to obtain new nanocomposites materials, composed of products based on alkaline phosphatase and natural biopolymers, collagen and chitosan gel. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), and water adsorption. The equilibrium Swelling was determined. The results obtained are promissing regarding the use of these supports to alkaline phosphatase (ALP) immobilization, the enzyme having a large practical use, especially in different skin tissue engineering.

Keywords

Fourier transform spectra; adsorption; bio-inspired materials; gels; infrared spectra; nanocomposites; proteins; swelling; tissue engineering; FTIR; Fourier transform infrared spectra; alkaline phosphatase immobilization; biopolymeric support; chitosan gel; collagen; enzyme; equilibrium swelling; nanocomposites; natural biopolymers; scaffolds; skin tissue engineering; water adsorption; Absorption; Biochemistry; Biomembranes; Nanocomposites; Plastics; Vibrations; FT-IR; alkaline phosphase; chitosan immobilization; collagen; water adsorption;

fLanguage

English

Publisher

ieee

Conference_Titel

Semiconductor Conference (CAS), 2014 International

Conference_Location

Sinaia

ISSN

1545-827X

Print_ISBN

978-1-4799-3916-9

Type

conf

DOI

10.1109/SMICND.2014.6966386

Filename

6966386