Title :
Immobilization of Alkaline phosphatase on biopolymeric support
Author :
Berteanu, A. Elena ; Tcacenco, Luminita ; Daniela Ionita, B. ; Demetrescu, Ioana
Author_Institution :
Nat. Inst. of R&D for Biol. Sci., Bucharest, Romania
Abstract :
This study is an investigation to obtain new nanocomposites materials, composed of products based on alkaline phosphatase and natural biopolymers, collagen and chitosan gel. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), and water adsorption. The equilibrium Swelling was determined. The results obtained are promissing regarding the use of these supports to alkaline phosphatase (ALP) immobilization, the enzyme having a large practical use, especially in different skin tissue engineering.
Keywords :
Fourier transform spectra; adsorption; bio-inspired materials; gels; infrared spectra; nanocomposites; proteins; swelling; tissue engineering; FTIR; Fourier transform infrared spectra; alkaline phosphatase immobilization; biopolymeric support; chitosan gel; collagen; enzyme; equilibrium swelling; nanocomposites; natural biopolymers; scaffolds; skin tissue engineering; water adsorption; Absorption; Biochemistry; Biomembranes; Nanocomposites; Plastics; Vibrations; FT-IR; alkaline phosphase; chitosan immobilization; collagen; water adsorption;
Conference_Titel :
Semiconductor Conference (CAS), 2014 International
Conference_Location :
Sinaia
Print_ISBN :
978-1-4799-3916-9
DOI :
10.1109/SMICND.2014.6966386