Title :
A New Sex Chromosome Marker, Similar to DNA Polymerase Type B, in the Platyfish Xiphophorus Maculatus
Author :
Zhou, Qingchun ; Froschauer, Alexander ; Schultheis, Christina ; Braasch, Ingo ; Scahartl, Manfred ; Jean-Nicolas, V.
Author_Institution :
Physiologische Chem. I, Univ. of Wurzburg, Wurzburg
Abstract :
A consensus sequence, encoding a putative DNA polymerase type B, was assembled from the sex determination region of Xiphophorus maculatus. This predicted protein, which is 1158aa in length, contains a DNA_pol_B_2 domain and a DTDS motif. The DNA polymerase type B gene has about 10 copies in the haploid X. maculatus, genome with one Y-specific copy. Interestingly, it has specific copies on the W chromosome in the X maculatus Usumacinta strain (sex determination with female heterogamety), which represent new markers for this type of sex chromosome in platyfish. This marker with W- and Y- specific copies suggests relationship between the different types of gonosomes and allows comparing male and female heterogameties in the platyfish. Further molecular analysis of the DNA polymerase type B gene in X maculatus will shed new light on the evolution of sex chromosomes in platyfish.
Keywords :
DNA; cellular biophysics; genetics; molecular biophysics; proteins; DNA polymerase type B; Xiphophorus maculatus; female heterogamety; genome; platyfish; protein; sex chromosome marker; sex determination; Assembly; Bioinformatics; Biological cells; Capacitive sensors; DNA; Encoding; Genomics; Polymers; Proteins; Sequences;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
DOI :
10.1109/ICBBE.2008.79
