An improved technology without mercury for cellular piercing

Fertilization performed by intracytoplasmic sperm injection(ICSI) using a piezo unit is recognized as the most practical technique not only for endangered species but also for humans. However what depresses us is the toxicity of mercury which may pose a bad influence on the health of humans and the competence of mouse oocytes. This limitation confines the use of this technique in hospitals and laboratories. What`s more, outcomes gained by the substitutes for mercury reducing the lateral oscillation is no match for mercury. Under this circumstance, a modified piezo-driven ICSI methodology, eliminating the application of mercury, is proposed in this paper used for the cleavage of mouse oocytes. By means of this modified methodology, a desirable result of cleavage for the cellular piercing was gained, a subtle lateral vibration of injection pipette smaller than its outer diameter obtained with the appropriate frequency and voltage. In view of the accomplishments we have acquired and the enhancements of manufacture for pipettes, this method will have a tremendous potential in hospitals and laboratories.