DocumentCode :
1654180
Title :
Using Fluorescent-Based AFLP to Analyze Genetic Diversity of Yellow-Seeded Brassica Napus L.
Author :
Yu, Chengyu ; Vyvadilova, Miroslava ; Leisova, Leona ; Ovesna, Jaroslava ; Kucera, Vratislav ; Hu, Shengwu ; Dotlacil, Ladislav
Author_Institution :
Coll. of Agronomy, Northwest A&F Univ., Yangling
fYear :
2008
Firstpage :
714
Lastpage :
717
Abstract :
A method for multiplex AFLP-PCR was employed to access the genetic diversity of yellow-seeded Brassica napus accessions originated from China, Czech Republic, and Poland. The AFLP-PCRs using three blended and fluorescent-labeled EcoRI+3 primers, AAC, AAG, and AC A, combing each time with one MseI+3primers CTC, CAC, CAG, CTG, CTT, and CTA, totally generated 723 peaks, of which 632 had high intension. 242 distinctly polymorphic markers among them were scored, with an average of 13.4 markers per primer pair. Based on either Dice, Jaccard, or Hamman similarity coefficients, the 31 accessions tested could be separated into Chinese, and non-Chinese group by UPGMA clustering, which corresponding well to the origins and pedigrees. Distinct geographical divergence was found among Chinese and non-Chinese yellow-seeded accessions. This AFLPs protocol were shown to be a reliable and quickly method for analyzing the genetic diversity than numeric taxonomy methods.
Keywords :
DNA; agriculture; fluorescence; genetics; molecular biophysics; China; Czech Republic; MseI+3primers; Poland; UPGMA clustering; amplified fragment length polymorphism; fluorescent-based AFLP-PCR; fluorescent-labeled EcoRI+3 primers; genetic diversity; polymorphic markers; taxonomy; yellow-seeded Brassica napus; Access protocols; Crops; DH-HEMTs; DNA; Educational institutions; Fluorescence; Genetics; Production; Taxonomy; Testing;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
Type :
conf
DOI :
10.1109/ICBBE.2008.174
Filename :
4535054
Link To Document :
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