Title :
Novel Gene Transduction Procedures in vivo Using the Epstein-Barr Virus-based Artificial Chromosomes
Author :
Mazda, Osam ; Kishida, Tsunao ; Asada, Hidetsugu ; Iida, Yasunori ; Tsunoda, Sei ; Oda, Yohei ; Shin-Ya, Masaharu ; Yoshikawa, Toshikazu ; Matsubara, Hiroaki ; Imanishi, Jiro
Author_Institution :
Dept. of Microbiol., Kyoto Prefectural Univ. of Medicine, Koto
Abstract :
Employment of Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene and oriP sequence grants characteristics of an artificial chromosome to DNA constructs, drastically improving transduction rate as well as expression level when transfected into mammalian cells, due not only to EBNA1-dependent nuclear transport of oriP-bearing DNA molecules but also to, albeit at a lesser extent, transcriptional up-regulation mediated by oriP/EBNA1. The present study demonstrates efficient transfer of the EBV-based artificial chromosomes in vivo into cardiac muscle by means of the electroporation and sonoporation
Keywords :
DNA; cellular biophysics; muscle; Epstein-Barr virus nuclear antigen 1; artificial chromosomes; cardiac muscle; electroporation; gene transduction procedure; mammalian cells; nuclear transport; oriP sequence; oriP-bearing DNA molecules; sonoporation; Biological cells; Cardiac disease; Cardiology; DNA; Immune system; In vitro; In vivo; Medical treatment; Muscles; Sequences;
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2006 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
1-4244-0717-6
Electronic_ISBN :
1-4244-0718-1
DOI :
10.1109/MHS.2006.320318
