Direct detection of DNA on gold structured planar substrates by Raman microscopy

Detection of DNA sequences is pivotal to many modern molecular diagnostic methods, but the ability to directly detect DNA sequences, without the need for signal amplification (such as by applying a polymerase chain reactions) is highly desirable. Here we investigate the potential for gold inverted pyramidal structures (also known as Klarite®) for DNA detection, as these simple chips containing the structured surface on a face could offer an improved format for DNA based diagnostic methods. Our strategy included optimization of the fabrication protocols to achieve flat gold surfaces within the inverted pyramidal surface and then a subsequent assessment of these substrates for direct DNA detection by Raman microscopy. These studies demonstrate for the first time the potential of these gold structured planar substrates for DNA analysis applications.