Hybrid digital holographic microscope for simultaneous measurement of 3D phase and 3D fluorescence distributions and its signal processing

Author

Xiangyu Quan ; Peng Xia ; Nitta, Kouich ; Matoba, Osamu ; Awatsuji, Yasuhiro

Author_Institution

Dept. of Syst. Sci., Kobe Univ., Kobe, Japan

fYear

2015

Firstpage

1

Lastpage

3

Abstract

A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and fluorescence images is proposed. The proposed microscopic system is designed by combination of a transmission type off-axis digital holographic configuration and a reflection type Fresnel incoherent holographic configuration, aimed for biological application. Owing to the single acquisition, signal processing which separates a superposed hologram is proposed.

Keywords

fluorescence; holography; optical information processing; optical microscopes; phase measurement; 3D fluorescence distributions measurement; 3D phase measurement; biological application; hybrid digital holographic microscope; reflection type Fresnel incoherent holographic configuration; signal processing; single acquisition; transmission type off-axis digital holographic configuration; Fluorescence; Holography; Image reconstruction; Lenses; Microscopy; Three-dimensional displays; Digital holography; Fluorescence microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Optics (WIO), 2015 14th Workshop on

Conference_Location

Kyoto

Type

conf

DOI

10.1109/WIO.2015.7206916

Filename

7206916