Title :
Hybrid digital holographic microscope for simultaneous measurement of 3D phase and 3D fluorescence distributions and its signal processing
Author :
Xiangyu Quan ; Peng Xia ; Nitta, Kouich ; Matoba, Osamu ; Awatsuji, Yasuhiro
Author_Institution :
Dept. of Syst. Sci., Kobe Univ., Kobe, Japan
Abstract :
A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and fluorescence images is proposed. The proposed microscopic system is designed by combination of a transmission type off-axis digital holographic configuration and a reflection type Fresnel incoherent holographic configuration, aimed for biological application. Owing to the single acquisition, signal processing which separates a superposed hologram is proposed.
Keywords :
fluorescence; holography; optical information processing; optical microscopes; phase measurement; 3D fluorescence distributions measurement; 3D phase measurement; biological application; hybrid digital holographic microscope; reflection type Fresnel incoherent holographic configuration; signal processing; single acquisition; transmission type off-axis digital holographic configuration; Fluorescence; Holography; Image reconstruction; Lenses; Microscopy; Three-dimensional displays; Digital holography; Fluorescence microscopy;
Conference_Titel :
Information Optics (WIO), 2015 14th Workshop on
Conference_Location :
Kyoto
DOI :
10.1109/WIO.2015.7206916
