Single-shot multispectral digital holographic microscopy

We propose a digital holographic microscopy technique capable of simultaneously sensing a multispectral 3D image with a single-shot exposure of a monochromatic image sensor. Multicolor and invisible 3D motion-picture images can be simultaneously recorded with a motion-picture recording of a monochromatic camera that has no wavelength filter. Multiwavelength information is spatially multiplexed on the image sensor plane and separately extracted in the spatial frequency domain. By introducing a large spatial carrier, multiple wavelength information is well separated and spatial information at each wavelength is obtained without the crosstalk noise. Holographic images at the wavelengths of 473, 532, 640, and 806 nm are obtained with a single-shot exposure of an image sensor by the microscopy.