Metrological analysis of the optical response to be acquired from an oligonucleotide library

Author

Cysewska-Sobusiak, Anna ; Boltrukiewicz, Michal

Author_Institution

Inst. of Electron. & Telecommun., Poznan Univ. of Technol., Poland

Volume

1

fYear

2002

fDate

6/24/1905 12:00:00 AM

Firstpage

391

Abstract

The subject of this paper relates to the measurable effects of light behavior in biological materials. The molecular complementarity of the two strands of the DNA double helix makes it possible to model changes in DNA sequences manifesting by related changes in their melting temperature, thermodynamic data and optical properties. This contribution is focused on the metrological problems connected with evaluation of parameters specifying the response of a virtual integrated oligonucleotide library. The presented analysis is concerned with the fluorescence signals to be obtained as a result of hybridization and detected by a CCD camera. Improving processing of acquired images is considering taking into account image smear that is still a fundamental limitation of the CCD detecting technique.

Keywords

CCD image sensors; DNA; biological effects of optical radiation; biological techniques; biomolecular effects of radiation; biothermics; fluorescence; melting; CCD camera; CCD detecting; DNA sequences changes modeling; biomeasurements; fluorescence signals; melting temperature; oligonucleotide biooptics; optical properties; thermodynamic data; virtual integrated oligonucleotide library; Biological materials; Biological system modeling; Biomedical optical imaging; Charge coupled devices; DNA; Libraries; Sequences; Signal analysis; Temperature; Thermodynamics;

fLanguage

English

Publisher

ieee

Conference_Titel

Instrumentation and Measurement Technology Conference, 2002. IMTC/2002. Proceedings of the 19th IEEE

ISSN

1091-5281

Print_ISBN

0-7803-7218-2

Type

conf

DOI

10.1109/IMTC.2002.1006873

Filename

1006873