Title :
Possible role of p21 and Hus1 in carbon nanotube induced genotoxicity
Author :
Tong, Liping ; Zhang, WenWen ; Hang, Haiying ; Wu, Lijun ; Xu, An
Author_Institution :
Key Lab. of Ion Beam Bioeng., Chinese Acad. of Sci., Hefei, China
Abstract :
The widely use of CNTs (single walled carbon nanotube and multi walled carbon nanotube) allows for potential exposure to this new nanomaterial. Most of the previous research on toxicology of CNTs have revealed that CNTs could induce genotoxicity via reactive oxygen species (ROS) indction. However the possible pathway of signal transduction was not completely understood. The aim of this work was to establish a possible role of p21 and hus1 gene in the impaired signaling pathway. Comparison of micronucleus fractions between wild type and p21-/-, hus1+/+ mouse embryo fibroblast (MEF) cells were performed in the experiment. The lower yield of micronucleus ratio in p21 knockout MEF cells indicated that p21 may contribute as an anti-apoptotic factor in the DNA damage signal transduction.
Keywords :
DNA; biochemistry; biomedical materials; carbon nanotubes; cellular transport; genetics; molecular biophysics; nanobiotechnology; toxicology; C; DNA damage; antiapoptotic factor; genotoxicity; hus1 gene; micronucleus fractions; mouse embryo fibroblast cells; multiwalled carbon nanotube; p21 gene; reactive oxygen species; signal transduction pathway; single walled carbon nanotube; Biomedical engineering; Carbon nanotubes; DNA; Embryo; Ion beams; Laboratories; Mice; Nanobioscience; Nuclear and plasma sciences; Physics;
Conference_Titel :
Nanoelectronics Conference (INEC), 2010 3rd International
Conference_Location :
Hong Kong
Print_ISBN :
978-1-4244-3543-2
Electronic_ISBN :
978-1-4244-3544-9
DOI :
10.1109/INEC.2010.5424809
