Novel integration method for chemical and enzyme sensor array by using microcontact printing

Author

Suzuki, Masayasu ; Nomura, Akitaka ; Yamamoto, Mizuki ; Minakuchi, Kenta ; Iribe, Yasunori

Author_Institution

Dept. of Electr. & Electron. Eng., Univ. of Toyama, Toyama, Japan

fYear

2010

Firstpage

301

Lastpage

306

Abstract

The fluorescence-based integrated chemical and enzyme sensor arrays on which two kinds of 10 μm i.d. sensor spots were arranged alternately on one chip, were developed by using microcontact printing. FITC, ruthenium complex and europium tetracycline was stamped onto a DLC sputtered glass slides for pH, oxygen and hydrogen peroxide sensors, respectively. After printing of oxygen sensor spots, pH sensor spots were stamped between oxygen sensor spots by using the same PDMS stamp. In the same way, glucose oxidase and lactate oxidase spots were stamped alternately onto H₂O₂ sensor spots. Each sensors in the integrated sensors showed specific responses independently.

Keywords

chemical sensors; enzymes; sensor arrays; soft lithography; enzyme sensor array; europium tetracycline; fluorescence-based integrated chemical; glucose oxidase; hydrogen peroxide sensor; integration method; lactate oxidase spot; microcontact printing; oxygen sensor spot; pH sensor spot; ruthenium complex; sputtered glass slide; Arrays; Biochemistry; Fluorescence; Ink; Sensors; Sugar;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2010 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4244-7995-5

Type

conf

DOI

10.1109/MHS.2010.5669540

Filename

5669540