Preparation of PPy film and its Application to Glucose Biosensor

Polypyrrole film was obtained by Chronopotentiometry method of electrochemical polymerization, with paratoluenesulfonic acid as supporting electrolyte and triple-electrode as working system. The polypyrrole film was characterized by infrared spectra (IR) and Scan electron microscope (SEM); And how its electrical conductivity affected by polymerization temperature and pyrrole concentration was studied. A glucose oxidase electrode with polypyrrole film as immobilizing carrier was prepared by a multiple-step electrochemical polymerization. The effects of reaction conditions such as temperature, solution pH, glucose concentration on the electrode bioelectrochemical response were discussed. Experimental results showed that the linear response range of glucose oxidase electrode was 1.0 times 10⁴ ~2.0 times 10^-2 mol/L and the detection limit was 1.0 times 10^-7mol/L. Its response time was 9 seconds.