Title :
Imaging cytochrome oxidation in hypothermically stored rat kidneys
Author :
Rorvik, Dawn A. ; Kelly, Jeff J. ; Burns, David H. ; Barlow, Clyde H.
Author_Institution :
Barlow Sci., Olympia, WA, USA
Abstract :
Spectroscopic analysis of mitochondrial cytochrome oxidation-reduction states is used to determine the respiratory integrity of hypothermically stored kidneys. One method uses a time-sharing fiber-optic spectrophotometer and the other uses a video-reflectance spectrometer to trace the reflectance changes of cytochrome c and cytochrome aa3 in the kidney cortex. Some experimental results are presented, showing that the metabolic function can be assessed in a way that is noninvasive, regionally specific, and technically simple
Keywords :
biological techniques and instruments; biothermics; kidney; proteins; biological technique; cytochrome aa3; cytochrome c; hypothermically stored rat kidneys; kidney cortex; metabolic function; mitochondrial cytochrome oxidation-reduction states; reflectance changes; respiratory integrity determination; time-sharing fiber-optic spectrophotometer; video-reflectance spectrometer; Band pass filters; Biochemistry; Electrons; Oxidation; Reflectivity; Spectroscopy; Steady-state; Surgery; Time sharing computer systems; Wavelength measurement;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.95903
