Title :
Potentiostatic deposition and detection of DNA on conductive nitrogen doped diamond-like carbon film electrode
Author :
Zeng, A. ; Samper, V. ; Tan, S.N. ; Poenar, D.P. ; Lim, T.M. ; Chew Kiat Heng
Author_Institution :
Sch. of Electr. & Electron. Eng., Nat. Univ. of Singapore, Singapore
Abstract :
We describe the electrochemical oxidation of deoxyribonucleic acid (DNA) at a conductive nitrogen doped diamond-like carbon (N-DLC) film electrode, and a procedure for reversible adsorption of DNA onto the N-DLC film electrode under potentiostatic control. N-DLC film electrode has well defined higher oxidation responses of guanine and adenine of denatured DNA compared to the response at a glassy carbon electrode. DNA can be deposited at the N-DLC film electrode when the electrode is positively charged and be released when the electrode is negatively charged. The N-DLC film electrode has an optimum deposition potential for DNA. The preliminary work shows that N-DLC film would be a novel electrode material for extraction and detection of DNA, which can be used for bio-micro-systems for DNA detection.
Keywords :
DNA; adsorption; biosensors; diamond-like carbon; electrochemical electrodes; electrochemical sensors; electrochemistry; nitrogen; oxidation; thin films; vacuum deposition; C:N; DNA adsorption; DNA detection; conductive nitrogen doped diamond-like carbon film electrode; deoxyribonucleic acid; electrochemical oxidation; glassy carbon electrode; potentiostatic deposition; Biological materials; Biosensors; Conductive films; DNA; Diamond-like carbon; Electrodes; Nitrogen; Optical device fabrication; Oxidation; Surface cleaning;
Conference_Titel :
TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003
Conference_Location :
Boston, MA, USA
Print_ISBN :
0-7803-7731-1
DOI :
10.1109/SENSOR.2003.1215293
