A 2D segmentation algorithm for the analisys of TBY-2 cells

TBY-2 cells are widely used in several applications and in particular in the study of programmed cell-death (PCD). However, automatic or semi-automatic computer-based tools supporting the specialist during the measurement process are still missing. In this paper we propose and test a semi-automatic tool for the segmentation of TBY-2 cell and the measurement of cytosol area. The algorithm has been designed in order to be easy to use and to have low complexity. Results obtained on a database of TBY-2 cells confirm that the algorithm can be effectively used by specialists in their daily work.