Title :
Screening of PCBs-degrading bacteria and enhanced bioremediation of soil under low-temperature condition
Author :
An Yong-lei ; Zhang Lan-ying ; Qiu Ming-ying ; Zhou Ai-xia ; Zhu Jin-rong ; Zhang Lei ; Ren He-jun
Author_Institution :
Key Lab. of Groundwater Resources & Environ., Jilin Univ., Changchun, China
Abstract :
Two low-temperature PCBs (polychlorinated biphenyls) -degrading strains were isolated from the long-term PCBs-contaminated soil and named strain P and strain Q which could utilize the PCBs as the sole carbon source and energy source. These two strains were identified by 16S rDNA sequence analysis. PCBs-degrading ability of these two strains was investigated by resting cell experiment and the bioremediation of PCBs-contaminated soil was simulated in laboratory. The results showed that strain P was initially identified as Sphingomonas sp. and strain Q was initially identified as Pseudomonas sp., both Strain P and strain Q had greater ability to degrade the low-chlorinated PCBs than the high-chlorinated PCBs. The highest removal rate of PCBs single-component by strain P was 77% while strain Q was 99%. The PCBs-degrading ability of strain Q was approximately 20%~30% higher than strain P. When two strains worked together in soil, the PCBs, especially the high-chlorinated PCBs could be removed more effectively. After adding surfactant hydroxypropyl-β-cyclodextrin to the bioremediation system of PCBs-contaminated soil, the growth of strain P did not change significantly while the biomass of strain Q increased obviously, and the removal rate of PCBs was twice more than not adding hydroxypropyl-β-cyclodextrin. Adding surfactant made the total removal rate of PCBs reached above 57%, which indicated that adding hydroxypropyl-β-cyclodextrin was conducive to the bioremediation of PCBs-contaminated soil.
Keywords :
DNA; biotechnology; contamination; microorganisms; soil; surfactants; water pollution; water treatment; PCB-degrading bacteria; Pseudomonas sp; Sphingomonas sp; biomass; bioremediation system; energy source; high-chlorinated PCB; long-term PCB-contaminated soil; low-chlorinated PCB; low-temperature PCB; polychlorinated biphenyls-degrading strain; rDNA sequence analysis; screening; sole carbon source; surfactant hydroxypropyl-β-cyclodextrin; total removal rate; Biomass; DNA; Degradation; Laboratories; Microorganisms; Soil; Strain; bioremediation; degrading bacteria; low-temperature; polychlorinated biphenyls;
Conference_Titel :
Water Resource and Environmental Protection (ISWREP), 2011 International Symposium on
Conference_Location :
Xi´an
Print_ISBN :
978-1-61284-339-1
DOI :
10.1109/ISWREP.2011.5893300