Analysis of flow cytometric data

Fluorescence Activated Cytometry is a research tool whose potential has yet to be fully exploited. Currently it is used for cluster hunting and cell counting. In this study, we separate cell clusters by identifying local minima in the cell density gradient to identify boundaries between cell types. These clusters were then analyzed for changes in intensity of four parameters marked by fluorescent antibody labels. The results are a statistical analysis of differences in the cell clusters which can be interpreted to understand what changes are occurring in the various cell populations on many levels.