The Effect of three-dimensional matrices on HLA expression by amniotic epithelial cells

Author

Khatami, Fatemeh ; Sattari, Mandana

Author_Institution

Dept. of Immunology, Shahid Beheshti Univ. of Med. Sci., Tehran, Iran

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Amniotic epithelial cells (AECs) with stem cell characteristics and unique immunological properties have created new hopes in regenerative medicine. Having differentiation potential into three germ layer cell type, AE cells are a precious cell source for clinical application. However, immunological changes of AE cells after culture and differentiation have limited their potential applications. The aim of this study was to determine matrix dimension effects on HLA (Human Lymphocyte Antigen) expression by these cells. Chitosan-gelatin three-dimensional (3D) (Scaffold) and two-dimensional (2D) matrices were used to evaluate MHC class I, HLA-DR and HLA-G expression in AE cells after 7 days culture. The results confirmed the effect of 3D and 2D systems on AECs immunogenicity especially on MHC class I (HLA-A, B, C) expression.

Keywords

cellular biophysics; gelatin; genetics; tissue engineering; 2D matrices; 3D matrices; 3D scaffold; AEC; HLA expression; amniotic epithelial cells; cell differentiation potential; chitosan-gelatin; germ layer cell type; human lymphocyte antigen expression; immunological property; matrix dimension effects; regenerative medicine; stem cell characteristics; Biomembranes; Cells (biology); Immune system; Pathogens; Stem cells; Three-dimensional displays; Tissue engineering; HLA; amniotic epithelial cell; chitosan; scaffold;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972836

Filename

6972836