The effects of cell density on viability within PEGDA hydrogel microspheres

Author

Perera, Davina ; Seethamraju, Deepika ; Olabisi, Ronke

Author_Institution

Dept. of Biomed. Eng., Rutgers Univ., New Brunswick, NJ, USA

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Cell microencapsulation can be used in tissue engineering as a scaffold or a physical barrier that provides immunoisolation for donor cells. Microencapsulation shields cells from the host immune system when they are delivered into the body for cell therapies. In order to maximize the delivery of therapeutic product per volume of microencapsulated cells, we must first optimize the seeding density of our hydrogel microspheres. In this study we microencapsulate mouse preosteoblast cells (MC3T3-E1) within poly(ethylene glycol) diacrylate hydrogel microspheres at low, medium, and high seeding densities in order to assess the viability of these cells as a function of cell density.

Keywords

biomedical materials; cellular biophysics; encapsulation; hydrogels; tissue engineering; PEGDA hydrogel microspheres; cell density effects; cell therapies; host immune system; immunoisolation; mouse preosteoblast cell microencapsulation; poly(ethylene glycol) diacrylate hydrogel microspheres; tissue engineering; Bones; Extracellular; Immune system; Media; Medical treatment; Proteins; Tissue engineering; hydrogel; microencapsulation; microspheres; tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972904

Filename

6972904