Title :
Quantitative optical biomarkers for non-invasive detection of cancerous transformation in live, 3D squamous epithelia
Author :
Pouli, Dimitra ; Varone, Antonio ; Xylas, Joanna ; Quinn, Kyle P. ; Hunter, Michael ; Georgakoudi, Irene ; McLaughlin-Drubin, Margaret E. ; Munger, Karl
Author_Institution :
Dept. of Biomed. Eng., Tufts Univ., Medford, MA, USA
Abstract :
Automated image analysis algorithms applied to two-photon excited fluorescence (TPEF) images, can non-invasively discern distinct, depth resolved metabolic and mitochondrial organization patterns in live 3D engineered epithelial tissues upon the onset of cancerous transformation.
Keywords :
biochemistry; biomedical optical imaging; cancer; cellular biophysics; data analysis; fluorescence; image resolution; medical image processing; tissue engineering; two-photon processes; TPEF image; automated image analysis algorithm; depth resolved metabolic organization pattern; depth resolved mitochondrial organization pattern; live 3D epithelial tissue engineering; live 3D squamous epithelia; noninvasive cancerous transformation detection; quantitative optical biomarker; two-photon excited fluorescence image; Biochemistry; Cancer; Fluorescence; Measurement; Optical imaging; Organizations; Three-dimensional displays; biomedical imaging; cancer; engineered epithelial tissues; metabolism; two-photon laser scanning fluorescence microscopy;
Conference_Titel :
Bioengineering Conference (NEBEC), 2014 40th Annual Northeast
Conference_Location :
Boston, MA
DOI :
10.1109/NEBEC.2014.6972909
