A multiplexed optical micro-impedance biosensor array for quantitaitive cancer drug assays

Optically based assays have traditionally been used to quantify cancer cell proliferation and cytotoxicity in response to chemotherapeutic agents. Cellular micro-impedance measurements provide complementary information that is a function of cellular adhesion and membrane properties. The ability to simultaneously acquire both optical and micro-impedance data has significant synergistic diagnostic potential. This study therefore proposes a multiplexed optical micro-impedance assay system compatible with routine pharmaceutical testing. Optically thin micro-impedance electrodes were designed for twenty-four and ninety-six well tissue culture plate readers. Electrode arrays were designed using micro-fabrication methods. The electrode arrays were then assembled into modified twenty-four and ninety-six well cell culture plates designed using computer aided design. Pharmaceutical research and personalized medicine applications are briefly discussed.