Title :
Near infrared fluorophore selection for lifetime-based FRET imaging
Author :
Sinsuebphon, Nattawut ; Lingling Zhao ; Intes, Xavier ; Bevington, Travis ; Ken, Abe ; Barroso, Margarida
Author_Institution :
Dept. of Biomed. Eng., Rensselear Polytech. Inst., Troy, NY, USA
Abstract :
Near-infrared fluorophore pairs were comparatively studied to perform quantitative lifetime-based FRET imaging. Comparison in terms of donor lifetime reduction, relative brightness and quantitative estimation of the FRETing donor fraction were carried out using a transferrin based assay applied to breast cancer cells in vitro experiments. The best pair, as defined as the brightest and leading to accurate estimation of FRETing component is identified as the most suitable for in vitro and in vivo pre-clinical application.
Keywords :
biomedical optical imaging; cancer; cellular biophysics; fluorescence; infrared imaging; molecular biophysics; proteins; FRETing donor fraction; breast cancer cells; donor lifetime reduction; in vitro preclinical application; in vivo preclinical application; near-infrared fluorophore pairs; near-infrared fluorophore selection; quantitative estimation; quantitative lifetime-based FRET imaging; relative brightness; transferrin based assay; Biomedical imaging; Breast cancer; Brightness; Fluorescence; In vitro; In vivo; FRET; lifetime imaging; time-resolved imaging; transferrin;
Conference_Titel :
Bioengineering Conference (NEBEC), 2014 40th Annual Northeast
Conference_Location :
Boston, MA
DOI :
10.1109/NEBEC.2014.6972941
