Title :
Design of a translational repression based RNA switch in mammalian cells
Author :
Sudrik, Chaitanya ; Arha, Manish ; Jicong Cao ; Kane, Ravi S. ; Schaffer, David V.
Author_Institution :
Dept. of Chem. & Biol. Eng., Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
Given the importance of molecular switches for the construction of artificial gene circuits, we developed a novel genetically encoded translational repression based switch that incorporates two copies of bovine immunodeficiency virus (BIV) TAR-based RNA hairpins in the 5´-untranslated region (UTR). We observed a 7-fold decrease in translational efficiency for the translational repression switch in the presence of BIV Tat ligand. We further characterized the effect of increasing amounts of BIV Tat peptide on the translational efficiency of the repression switch.
Keywords :
RNA; cellular biophysics; genetics; molecular biophysics; proteins; 5´-untranslated region; 7-fold decrease; BIV Tat ligand; BIV Tat peptide; artificial gene circuit construction; bovine immunodeficiency virus TAR-based RNA hairpins; genetically encoded translational repression based switch; mammalian cells; molecular switches; translational efficiency; translational repression based RNA switch; translational repression switch; Bovine; Fluorescence; Peptides; Proteins; RNA; Switches; Switching circuits; BIV Tat; RNA switch; TAR; mRNA; synthetic biology; translation;
Conference_Titel :
Bioengineering Conference (NEBEC), 2014 40th Annual Northeast
Conference_Location :
Boston, MA
DOI :
10.1109/NEBEC.2014.6972948
