Lab-on-a-disk immunoassay for ocular diagnostics

Author

Walsh, David I. ; Murthy, Shashi K. ; Hahn, Paul S. ; Jaffe, Glenn J. ; Sommer, Gregory J. ; Schaff, Ulrich Y.

Author_Institution

Northeastern Univ., Boston, MA, USA

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Monitoring of angiogenic cytokines in the eyes of patients with diabetic retinopathy (DR) and age-related macular degeneration (AMD) is crucial to confirm efficacy of treatment. We present a novel “lab-on-a-disk” immunoassay for monitoring vascular endothelial growth factor in the eye with potential for deployment at the ophthalmologist´s office as a point-of-care diagnostic. Using rudimentary sedimentation principles, the immunoassay can be completed in a two-step method within 16-18 minutes. The platform is comparable in sensitivity to conventional ELISA. Adoption of this platform at the point-of-care will facilitate accurate dosing of anti-angiogenic medication used to treat DR and AMD as well as identify non-responders.

Keywords

bioMEMS; diseases; eye; lab-on-a-chip; microfluidics; patient diagnosis; patient monitoring; ELISA; age-related macular degeneration; angiogenic cytokines monitoring; anti-angiogenic medication; diabetic retinopathy; eyes; lab-on-a-disk immunoassay; ocular diagnostics; point-of-care diagnostic; rudimentary sedimentation principles; vascular endothelial growth factor; Biochemistry; Diabetes; Fluorescence; Immune system; Microfluidics; Standards; Time measurement; AMD; DR; ELISA; VEGF; lab-on-a-disk; microfluidics; ocular disease;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972966

Filename

6972966