Cellular adhesion and protein adsorption to P(MEO2MA-co-OEGMA) polymer substrates

Author

Warrener, Matthew ; Anderson, Christopher R.

Author_Institution

Dept. of Chem. & Biomol. Eng., Lafayette Coll., Easton, PA, USA

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Thermoresponsive polymers (TRP) are attractive substrates for cell culture, as adherent cells are mechanically released from the polymer surface when the temperature is lowered below the lower critical solution temperature (LCST). In this study, cell adhesion and serum protein adsorption to P(MEO₂MA-co-OEGMA) (PMO) polymer substrates was investigated. L-929 cell adhesion to PMO surfaces was markedly lower that the adhesion to tissue culture plastic surfaces (TCP) after 6 hours. The density of adsorbed serum proteins to PMO surfaces was significantly higher (2.3 fold) than TCP surfaces after incubation with cell culture media with 10% fetal bovine serum (FBS). Selective adsorption of extracellular matrix proteins may further enhance adhesion to PMO surfaces.

Keywords

adhesion; adsorption; biochemistry; biological tissues; biomechanics; biomedical materials; cellular biophysics; molecular biophysics; polymer blends; proteins; L-929 cell adhesion-PMO surfaces; L-929 cell adhesion-tissue culture plastic surfaces; P(MEO₂MA-co-OEGMA) polymer substrates; extracellular matrix protein adsorption; fetal bovine serum; lower critical solution temperature; serum protein adsorption; thermoresponsive polymers; time 6 hour; Adhesives; Adsorption; Media; Polymers; Proteins; Substrates; biomaterials; cell adhesion; protein adsorption; thermoresponsive polymers;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972974

Filename

6972974