DocumentCode :
171696
Title :
Defatting heptocytes under flow
Author :
Yarmush, Joshua ; Yarmush, Gabriel ; Nativ, Nir ; Berthiaume, Francois
Author_Institution :
Dept. of Biomed. Eng., State Univ. of New Jersey, Piscataway, NJ, USA
fYear :
2014
fDate :
25-27 April 2014
Firstpage :
1
Lastpage :
2
Abstract :
There is a critical shortage of transplantable livers in both the US and the world. One method to increase the donor pool is to develop methodology to recondition extended criteria donor grafts, a large portion of which are moderate to severe macrosteatotic livers. Transplantation of these livers often leads to primary nonfunction caused by an increased susceptibility to the effects of ischemia reperfusion injury that result from the harvesting, transportation, and transplantation of the liver. Our lab has developed a novel cocktail of defatting reagents that can, over a period of two days, render hepatocytes lean and with good cell viability and function. Despite this accomplishment, in order to be feasibly performed in a clinical setting, the defatting process must be completed in a matter of hours. The current project focuses on understanding the differences between defatting in a static versus flow environment and then using this information to develop the ideal parameters for defatting whole organs. Our hypothesis in the current work is that using flow and the appropriate defatting agents, steatotic hepatocytes can be defatted in a clinically relevant time of hours, without decreasing cell viability or function. In order to test this hypothesis, a perfusion reactor was constructed which holds microscope slides that can be seeded with fatty hepatocytes. Perfusion of defatting media was performed at different flow rates over a six hour period. Results showed that within a six hour period, significant defatting (as compared to static cultures) was observed at both 2.0 and 4.0 ml/min as indicated by oil red O staining. Current experiments are focused on further evaluation of hepatocyte viability and function (e.g. albumin, urea, and cytochrome P450 function). These types of parametric experiments will form the basis for future perfusions with whole organs from obese rats.
Keywords :
cellular biophysics; haemorheology; liver; patient treatment; cell function; cell viability; defatting media perfusion; defatting reagents; fatty hepatocytes; hepatocyte function; hepatocyte viability; heptocytes defatting; ischemia reperfusion injury; liver transplantation; microscope slides; obese rats; oil red O staining; perfusion reactor; steatotic hepatocytes; Inductors; Injuries; Lipidomics; Liver; Microscopy; Rats; Standards; Macrosteatosis; defatting; flow device; hepatocytes; liver function;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioengineering Conference (NEBEC), 2014 40th Annual Northeast
Conference_Location :
Boston, MA
Type :
conf
DOI :
10.1109/NEBEC.2014.6972985
Filename :
6972985
Link To Document :
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