DocumentCode :
171722
Title :
Fabrication of cell patches using scaffolds with a hexagonal array of interconnected pores (SHAIPs)
Author :
Zhang, Yu Shrike ; Younan Xia
Author_Institution :
Med. Sch., Dept. of Med., Harvard Univ., Boston, MA, USA
fYear :
2014
fDate :
25-27 April 2014
Firstpage :
1
Lastpage :
2
Abstract :
Cell patches are widely used for healing injuries on the surfaces or interfaces of tissues. Here we present a novel class of porous scaffolds made of poly(D, L-lactic-co-glycolic) acid for fabricating cell patches. The scaffolds have a single layer of spherical pores that are arranged in a unique hexagonal pattern and are therefore referred to as “scaffolds with a hexagonal array of interconnected pores (SHAIPs)”. SHAIPs contain both uniform pores and interconnecting windows that can facilitate the exchange of biomacromolecules, ensure homogeneous cell seeding, and promote cell migration. As a proof-of-concept demonstration, we have created skeletal muscle patches with a thickness of approximately 150 μm using SHAIPs. The myoblasts seeded in the scaffolds maintained high viability and were able to differentiate into multi-nucleated myotubes. Moreover, neovasculature could efficiently develop into the patches upon subcutaneous implantation in vivo.
Keywords :
biomedical materials; bone; cell motility; injuries; materials preparation; molecular biophysics; muscle; patient treatment; polymers; porous materials; tissue engineering; SHAIP; biomacromolecule exchange; cell migration; cell patch fabrication; healing injuries; homogeneous cell seeding; interconnecting windows; multinucleated myotubes; myoblasts; neovasculature; poly(D, L-lactic-co-glycolic) acid; porous scaffolds; proof-of-concept demonstration; scaffolds with a hexagonal array of interconnected pores; single layer; size 150 mum; skeletal muscle patches; spherical pores; subcutaneous implantation in vivo; tissue interfaces; uniform pores; unique hexagonal pattern; Arrays; Biomedical imaging; Blood vessels; Educational institutions; Fabrication; In vivo; Muscles; cell patch; inverse opal scaffolds; regenerative engineering; skeletal muscle; uniform;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioengineering Conference (NEBEC), 2014 40th Annual Northeast
Conference_Location :
Boston, MA
Type :
conf
DOI :
10.1109/NEBEC.2014.6972998
Filename :
6972998
Link To Document :
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