MEMS-based sonoporation devices for intratumoral gene therapy

Author

Siu, T. ; Trivedi, D.M. ; Chiao, M. ; Rohling, R.

Author_Institution

Dept. of Mech. Eng., British Columbia Univ., Vancouver, BC, Canada

Volume

1

fYear

2005

Firstpage

205

Abstract

This paper presents a miniature device for intratumoral delivery of oligonucleotides (ASO). A PZT plate (1 cm² × 0.75mm) is used to transfect cells using cavitation-induced sonoporation. Both human umbilical vein endothelial cells (HUVEC) and human prostate cancer cells (PC3) are investigated in vitro. Preliminary results show that after cavitation-induced sonoporation, the transfection rate for HUVEC increases by 10% compared to controls (p<0.01). For PC3, the transfection rate increases by 7% compared to controls (p<0.01).

Keywords

biomedical equipment; biomedical ultrasonics; cavitation; cellular biophysics; genetics; micromechanical devices; patient treatment; 0.75 mm; 1 cm; HUVEC; MEMS-based sonoporation devices; PC3; PZT plate; cavitation-induced sonoporation; cell transfection; human prostate cancer cells; human umbilical vein endothelial cells; intratumoral gene therapy; oligonucleotides; Actuators; Biomembranes; Cancer; Cells (biology); DNA; Gene therapy; Humans; In vitro; Medical treatment; Ultrasonic imaging;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on

Print_ISBN

0-7803-8994-8

Type

conf

DOI

10.1109/SENSOR.2005.1496394

Filename

1496394