Title :
Ion-selective microelectrode array for intracellular detection on chip
Author :
Guenat, O.T. ; Xiao Jie Wang ; Dufour, J.-F. ; van der Wal, P. ; Morf, W.E. ; de Rooij, N.F. ; Koudelka-Hep, M.
Author_Institution :
Inst. of Microtechnol., Neuchatel Univ., Switzerland
Abstract :
The design and fabrication of an Ion-Selective Electrode (ISE) array platform for in-vitro intracellular recording are presented. The platform is composed of two parts: 1) a glass chip with microchannels whose bottom part is patterned with a silver layer and 2) a silicon chip in with an array of 30 /spl mu/m long silicon nitride micropipettes with an inner diameter of 1.7 /spl mu/m long silicon nitride micropipettes with an inner diameter of 1.7 /spl mu/m are structured. The Ca/sup 2+/ selective membrane is based on the neutral carrier ionophore ETH 129. To be compatible with hepatocyte cell cultures the 24 ISEs are arranged in a 2/spl times/8 array with 150 /spl mu/m spacing and four additional electrodes located on both sides of the array at 300 /spl mu/m.
Keywords :
microelectrodes; micromechanical devices; silicon compounds; silver; silver compounds; 1.7 micron; 150 micron; 30 micron; 300 micron; Ag-AgCl; SiN; electrodes; glass chip; hepatocyte cell cultures; intracellular detection; intracellular recording; ion-selective microelectrode array; microchannels; neutral carrier ionophore; selective membrane; silicon chip; silicon nitride micropipettes; silver layer; Actuators; Biomembranes; Electrodes; Fabrication; Fluorescence; Glass; In vitro; Microchannel; Sensor arrays; Silicon;
Conference_Titel :
TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003
Conference_Location :
Boston, MA, USA
Print_ISBN :
0-7803-7731-1
DOI :
10.1109/SENSOR.2003.1216952
