Title :
On/off control of biomolecular motors in a microfluidic device
Author :
Yokokawa, R. ; Takeuchi, S. ; Kon, T. ; Nishiura, M. ; Edamatsu, M. ; Sutoh, K. ; Fujita, H.
Author_Institution :
CIRMM, Tokyo Univ., Japan
Abstract :
We have successfully realized an on/off control of biomolecular linear motors in a polydimethyl siloxane (PDMS) chamber sealed with a cover glass. A linear biomolecular motor system, microtubule-kinesin system, is controlled by injecting the optimized concentrations of adenosine triphosphate (ATP) for the activation and hexokinase for the stoppage. Not only control from on to off or off to on, the repetitive injection of ATP and hexokinase proves that kinesin molecules can move and stop alternatively once they bind to microtubules.
Keywords :
biomolecular electronics; microfluidics; molecular biophysics; polymers; adenosine triphosphate; biomolecular linear motor; glass; hexokinase; microfluidic device; microtubule kinesin system; on/off control; polydimethyl siloxane chamber; Buffer storage; Chemicals; Glass; Lenses; Microfluidics; Micromotors; Microscopy; Polymers; Rails; Transducers;
Conference_Titel :
TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003
Conference_Location :
Boston, MA, USA
Print_ISBN :
0-7803-7731-1
DOI :
10.1109/SENSOR.2003.1216968
