Electroformation of giant liposomes in microfluidic channels

This paper describes a method to produce giant liposomes in microfluidic channels. To prepare the liposomes, we have investigated two different methods: electroformation and conventional gentle-hydration. We found that the liposomes produced by the conventional method were relatively small size, and several liposomes were enclosed inside another liposome. On the other hand, the liposomes formed by electroformation were mostly giant liposomes and did not enclose liposomes inside. Using microfluidic channels with electroformation method, we have succeeded in enclosing different types of the nano/micro materials into giant liposomes in the same microfluidic device simultaneously.