Title :
Polarization-sensitive second harmonic confocal imaging of biological tissues
Author :
Kim, Beop-Min ; Stoller, Patrick ; Reiser, Karen ; Rubenchik, Alexander ; Da Silva, Luiz
Author_Institution :
Med. Technol. Program, Lawrence Livermore Nat. Lab., CA, USA
Abstract :
Among the many collagen-rich tissues, we studied rat-tail tendon in detail since it is composed of highly-organized type I collagen fibrils. We devised a confocal microscope that measures the second harmonic light generated from rat-tail tendon. Since SHG is sensitive to the linear polarization of the excitation pulse, a mathematical model was developed to investigate the relationship between linear polarization, the direction of the collagen fibrils, and the intensity of the generated second harmonic light. Also, the effect of collagen organization on the second harmonic intensity was studied
Keywords :
biomedical imaging; laser applications in medicine; optical harmonic generation; proteins; biological tissues; biomedical imaging; collagen organization; collagen-rich tissues; confocal microscope; generated second harmonic light; highly-organized type I collagen fibrils; linear polarization; polarization-sensitive second harmonic confocal imaging; rat-tail tendon; solid lasers; Biological tissues; Biomedical imaging; Biomedical optical imaging; Laser beams; Nonlinear optics; Optical harmonic generation; Optical materials; Polarization; Pump lasers; Tendons;
Conference_Titel :
Lasers and Electro-Optics Society 2000 Annual Meeting. LEOS 2000. 13th Annual Meeting. IEEE
Conference_Location :
Rio Grande
Print_ISBN :
0-7803-5947-X
DOI :
10.1109/LEOS.2000.890748
