Three-dimensional bio-micromanipulation under the microscope

We develop a new micromanipulation system for anatomical operation of the micro-object such as an embryo, cell, and microbe. The image of the microscope is two-dimensional, so it is hard to manipulate the target in the 3D space. To improve the manipulation work, we proposed a 3D bio-micromanipulation system combined with a virtual reality (VR) space. We propose a 3D object modeling method for presenting 3D visual information to the operator and improve the operation environment. In this system, we still have difficulty in changing the orientation of the microscopic object by manipulating a mechanical manipulator. The bio-aligner proposed is a micro device for the posture control of an object. We develop a 2D bio-aligner by microfabrication. Here we show its fabrication process and a basic rotating experiment with yeast cells.