Title :
Reconstruction of Cellular Processes in Nanoscale Artificial Organelles Solvent-free Incorporation of Proteins into Block Copolymers
Author :
Choi, Hyo-Jick ; Montemagno, Carlo D.
Author_Institution :
Department of Bioengineering, UCLA, 420 Westwood Plaza, Room 7523 Boelter Hall, PO Box 951600, Los Angeles, CA 90095-1600, USA, hyochung@ucla.edu
Abstract :
Development of a solvent-free method for protein-incorporated polymersome (proteopolymersome) formation is regarded as a major challenge in replicating cellular functions in engineered cellular mimetic systems. Presented is the first successful functional proteopolymersomes made without organic solvents. For this purpose, we reconstructed in vitro proton-powered ATP synthesis using engineered artificial organelles, BR/F0F1-ATP synthase reconstituted polymersomes. This research is expected to enhance the potential applications of synthetic artificial membranes for future hybrid protein/polymer systems.
Keywords :
artificial organelle; polymersome; self-assembly; triblock copolymer; Biological materials; Biomembranes; Biomimetics; Cells (biology); Lipidomics; Polymers; Protein engineering; Protons; Self-assembly; Solvents; artificial organelle; polymersome; self-assembly; triblock copolymer;
Conference_Titel :
Nanotechnology, 2006. IEEE-NANO 2006. Sixth IEEE Conference on
Print_ISBN :
1-4244-0077-5
DOI :
10.1109/NANO.2006.247593
