Title :
Fragmental Frequency Analysis Method to Estimate Electrical Cell Parameters From Bioimpedance Study
Author :
Das, Divya ; Kamil, Farhan Ahmad ; Agrawal, Sanjay ; BISWAS, KAMANASHIS ; Das, S.
Author_Institution :
Dept. of Electr. Eng., Indian Inst. of Technol. Kharagpur, Kharagpur, India
Abstract :
This paper introduces an alternate approach utilizing the fragmental frequency analysis method for analyzing bioimpedance data to estimate electrical cell parameters. Impedance of cervical cancer cells (HeLa) in phosphate buffer saline media is measured using an electric cell-substrate impedance sensing (ECIS) device and impedance analyzer. The measured impedance data were visualized by modeling an equivalent electrical circuit of the system considering the dominancy of individual parameters of the ECIS system and analyzed in different frequency zones. The present approach eliminates the convergence inaccuracy in fitting the experimental impedance data with fitting software arising due to invalid initial conditions, and a large number of data points. This method provides high-frequency characterization, modeling of ECIS system, knowledge of effect of ECIS model parameters with frequency, and an alternate way to calculate model parameters.
Keywords :
bioelectric phenomena; cancer; cellular biophysics; convergence; electric impedance measurement; gynaecology; ECIS; HeLa; bioimpedance study; cervical cancer cells; convergence inaccuracy; data points; electric cell-substrate impedance sensing device; electrical cell parameters; equivalent electrical circuit modeling; fitting software; fragmental frequency analysis method; impedance analyzer; phosphate buffer saline media; Capacitance; Electrodes; Equivalent circuits; Frequency measurement; Impedance; Integrated circuit modeling; Media; Bioimpedance; Bode--Nyquist plot; Bode??Nyquist plot; ECIS; fragmental frequency analysis; impedance spectrum;
Journal_Title :
Instrumentation and Measurement, IEEE Transactions on
DOI :
10.1109/TIM.2014.2303553
