Effect of reference electrode in the nanoFET (field-effect transistor)-based biosensor experiment

Author

Yeong-Tai Seo ; Kuk Jin Jang ; Min-Ho Lee ; Woo-Kyeong Seong ; Kook-Nyung Lee ; Yong-Kweon Kim

Author_Institution

Sch. of Electr. Eng. & Comput. Sci., Seoul Nat. Univ., Seoul, South Korea

Volume

9

Issue

12

fYear

2014

fDate

12 2014

Firstpage

874

Lastpage

876

Abstract

The reference electrode in an electrolyte solution affects the surface potential of the nanoFET (field-effect transistor) channel which can result in a false signal to be measured in the FET channel during bio- and chemical sensing experiments. In this reported work, it was demonstrated that different sensing results that were obtained were affected by the choice of the reference electrode through a pH and ion concentration sensing experiment. Three types of reference electrodes were compared: the SCE (saturated calomel reference electrode), the Ag/AgCl tube and the integrated Ag/AgCl electrode. The pH sensitivity equivalent to the surface potential was evaluated: 32.3, 34.6 and 16.3 mV/pH in the case of the SCE, the refillable tube type Ag/AgCl and the integrated Ag/AgCl, respectively.

Keywords

biochemistry; biosensors; electrochemical electrodes; electrochemical sensors; electrolysis; field effect transistors; pH; silver; silver compounds; surface potential; Ag-AgCl; Ag-AgCl tube; chemical sensor; electrolyte solution; field-effect transistor channel; field-effect transistor-based biosensor; integrated Ag-AgCl electrode; ion concentration; nanoFET based-biosensor; nanoFET channel; pH sensitivity; reference electrode; saturated calomel reference electrode; surface potential;

fLanguage

English

Journal_Title

Micro & Nano Letters, IET

Publisher

iet

ISSN

1750-0443

Type

jour

DOI

10.1049/mnl.2014.0405

Filename

6992359