Design and evaluation of a microcantilever aptasensor

Author

Yang Choon Lim ; Kouzani, Abbas Z. ; Wei Duan ; Dai, Xiujuan J. ; Kaynak, Akif ; Mair, Douglas

Author_Institution

Melbourne Centre for Nanofabrication, Melbourne, VIC, Australia

fYear

2014

fDate

1-5 June 2014

Firstpage

221

Lastpage

224

Abstract

Aptamers enhance flexibility in biosensor design. An aptasensor employs aptamers as a biological recognition element for biosensing. This paper presents design, fabrication, and evaluation of a microcantilever aptasensor. To identify and avoid potential bottlenecks in the aptasensor design, the parameters of the aptasensor are investigated through modelling and simulation. Next, thin SU-8 microcantilevers are fabricated to form the aptasensor. Characterization of the fabricated aptasensor is presented. Next, a plasma-based surface funtionalisation method is used to immobilize aptamers on the atasensor. Finally, an evaluation of the performance of the aptasensor is performed through detection of thrombin molecules. The evaluation results are presented and discussed.

Keywords

biological techniques; biosensors; cantilevers; microfabrication; microsensors; aptamers immobilization; biological recognition element; biosensor design; microcantilever aptasensor; plasma-based surface funtionalisation method; thin SU-8 microcantilever; thrombin molecule detection; Laser beams; Plasmas; Sensitivity; Sensors; Surface cleaning; Surface morphology; aptasensor; detection; microcantilever;

fLanguage

English

Publisher

ieee

Conference_Titel

Circuits and Systems (ISCAS), 2014 IEEE International Symposium on

Conference_Location

Melbourne VIC

Print_ISBN

978-1-4799-3431-7

Type

conf

DOI

10.1109/ISCAS.2014.6865105

Filename

6865105